cell sorting greiner bioone (Greiner Bio)
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cell sorting greiner bioone
Cell Sorting Greiner Bioone, supplied by Greiner Bio, used in various techniques. Bioz Stars score: 96/100, based on 795 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/cell+sorting+greiner+bioone/Leucosep+Tube+50+Ml+Pp+30%2F115+Mm/pm35045291-245-237-239
Average 96 stars, based on 795 article reviews
Cell Sorting Greiner Bioone, supplied by Greiner Bio, used in various techniques. Bioz Stars score: 96/100, based on 795 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/cell+sorting+greiner+bioone/Leucosep+Tube+50+Ml+Pp+30%2F115+Mm/pm35045291-245-237-239
Average 96 stars, based on 795 article reviews
cell sorting greiner bioone - by Bioz Stars,
2026-09
96/100 stars
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Isolation:Article Title: The endothelial diapedesis synapse regulates transcellular migration of human T lymphocytes in a CX3CL1- and SNAP23-dependent manner. Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Swine Anti-Rabbit Immunoglobulins/HRP (affinity isolated), WB Dako Cat#P0399 Biological samples Fresh blood, in heparin tubes, from voluntary healthy donors Sanquin Blood Supply, Amsterdam, NL N/A Buffy coats, from the transfusion service Sanquin Blood Supply, Amsterdam, NL N/A Chemicals, peptides, and recombinant proteins Brefeldin A, BioXtra, for molecular biology Sigma Aldrich Cat#B6542 Recombinant human tumor necrosis factor a, TNF-a Peprotech Cat#300-01A; Accession Number: P01375 Pertussis toxin from Bordetella pertussis Merck, formerly from Sigma Aldrich Cat#P7208 Phalloidin Alexa Fluor 488 Thermo Fisher Scientific Cat# A12379 VybrantTM DiD Cell-Labeling Solution Thermo Fisher Scientific Cat#V22887 HOECHST 33342 Thermo Fisher Scientific Cat#H1399 Critical commercial assays Proteome profiler human chemokine kit Biotechne, RnD Systems Cat#ARY017 Experimental models: Cell lines HUVEC, human umbilical vein endothelial cells, pooled Lonza, Bornem, Beligum Cat#C2519A Oligonucleotides Human SNAP23, (NM_003825, NM_ 130798), siRNA1 Qiagen GeneGlobeID: SI00056448 Human SNAP23, (NM_003825, NM_ 130798), siRNA2 Qiagen GeneGlobeID: SI00056455 Negative Control siRNA, human Qiagen GeneGlobeID: 1022076 Recombinant DNA pmEGFP-N1-Lifeact Jaap van Buul, Sanquin, Amsterdam, NL N/A pLV-mScarlet_CAAX Jaap van Buul, Sanquin, Amsterdam, NL N/A pLV_ICAM-1_GFP Bram van Steen, Sanquin, Amsterdam, NL N/A Software and algorithms Fiji/ImageJ, ImageJ v1.53 (Schindelin et al., 2012) https://doi.org/10.1038/nmeth.2019 Graphpad Prism 9.1.0 (221) Graphpad Software, LLC https://www.graphpad.com/scientific- software/prism/ FlowJo v10.8 BD https://www.flowjo.com/solutions/flowjo Imaris 9.7.2 Bitplane https://imaris.oxinst.com/ Other Ficoll-Paque PLUS, density gradient media GE Healthcare Cat#17144003 CD8+ T cell isolation kit Miltenyi Biotech Cat#130-045-201 EGM-2 medium Promocell Cat#C-22011 EGM-2 supplement kit Promocell Cat#C-39216 Leukosep tubes, Western Blot:Article Title: The endothelial diapedesis synapse regulates transcellular migration of human T lymphocytes in a CX3CL1- and SNAP23-dependent manner. Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Swine Anti-Rabbit Immunoglobulins/HRP (affinity isolated), WB Dako Cat#P0399 Biological samples Fresh blood, in heparin tubes, from voluntary healthy donors Sanquin Blood Supply, Amsterdam, NL N/A Buffy coats, from the transfusion service Sanquin Blood Supply, Amsterdam, NL N/A Chemicals, peptides, and recombinant proteins Brefeldin A, BioXtra, for molecular biology Sigma Aldrich Cat#B6542 Recombinant human tumor necrosis factor a, TNF-a Peprotech Cat#300-01A; Accession Number: P01375 Pertussis toxin from Bordetella pertussis Merck, formerly from Sigma Aldrich Cat#P7208 Phalloidin Alexa Fluor 488 Thermo Fisher Scientific Cat# A12379 VybrantTM DiD Cell-Labeling Solution Thermo Fisher Scientific Cat#V22887 HOECHST 33342 Thermo Fisher Scientific Cat#H1399 Critical commercial assays Proteome profiler human chemokine kit Biotechne, RnD Systems Cat#ARY017 Experimental models: Cell lines HUVEC, human umbilical vein endothelial cells, pooled Lonza, Bornem, Beligum Cat#C2519A Oligonucleotides Human SNAP23, (NM_003825, NM_ 130798), siRNA1 Qiagen GeneGlobeID: SI00056448 Human SNAP23, (NM_003825, NM_ 130798), siRNA2 Qiagen GeneGlobeID: SI00056455 Negative Control siRNA, human Qiagen GeneGlobeID: 1022076 Recombinant DNA pmEGFP-N1-Lifeact Jaap van Buul, Sanquin, Amsterdam, NL N/A pLV-mScarlet_CAAX Jaap van Buul, Sanquin, Amsterdam, NL N/A pLV_ICAM-1_GFP Bram van Steen, Sanquin, Amsterdam, NL N/A Software and algorithms Fiji/ImageJ, ImageJ v1.53 (Schindelin et al., 2012) https://doi.org/10.1038/nmeth.2019 Graphpad Prism 9.1.0 (221) Graphpad Software, LLC https://www.graphpad.com/scientific- software/prism/ FlowJo v10.8 BD https://www.flowjo.com/solutions/flowjo Imaris 9.7.2 Bitplane https://imaris.oxinst.com/ Other Ficoll-Paque PLUS, density gradient media GE Healthcare Cat#17144003 CD8+ T cell isolation kit Miltenyi Biotech Cat#130-045-201 EGM-2 medium Promocell Cat#C-22011 EGM-2 supplement kit Promocell Cat#C-39216 Leukosep tubes, Recombinant:Article Title: The endothelial diapedesis synapse regulates transcellular migration of human T lymphocytes in a CX3CL1- and SNAP23-dependent manner. Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Swine Anti-Rabbit Immunoglobulins/HRP (affinity isolated), WB Dako Cat#P0399 Biological samples Fresh blood, in heparin tubes, from voluntary healthy donors Sanquin Blood Supply, Amsterdam, NL N/A Buffy coats, from the transfusion service Sanquin Blood Supply, Amsterdam, NL N/A Chemicals, peptides, and recombinant proteins Brefeldin A, BioXtra, for molecular biology Sigma Aldrich Cat#B6542 Recombinant human tumor necrosis factor a, TNF-a Peprotech Cat#300-01A; Accession Number: P01375 Pertussis toxin from Bordetella pertussis Merck, formerly from Sigma Aldrich Cat#P7208 Phalloidin Alexa Fluor 488 Thermo Fisher Scientific Cat# A12379 VybrantTM DiD Cell-Labeling Solution Thermo Fisher Scientific Cat#V22887 HOECHST 33342 Thermo Fisher Scientific Cat#H1399 Critical commercial assays Proteome profiler human chemokine kit Biotechne, RnD Systems Cat#ARY017 Experimental models: Cell lines HUVEC, human umbilical vein endothelial cells, pooled Lonza, Bornem, Beligum Cat#C2519A Oligonucleotides Human SNAP23, (NM_003825, NM_ 130798), siRNA1 Qiagen GeneGlobeID: SI00056448 Human SNAP23, (NM_003825, NM_ 130798), siRNA2 Qiagen GeneGlobeID: SI00056455 Negative Control siRNA, human Qiagen GeneGlobeID: 1022076 Recombinant DNA pmEGFP-N1-Lifeact Jaap van Buul, Sanquin, Amsterdam, NL N/A pLV-mScarlet_CAAX Jaap van Buul, Sanquin, Amsterdam, NL N/A pLV_ICAM-1_GFP Bram van Steen, Sanquin, Amsterdam, NL N/A Software and algorithms Fiji/ImageJ, ImageJ v1.53 (Schindelin et al., 2012) https://doi.org/10.1038/nmeth.2019 Graphpad Prism 9.1.0 (221) Graphpad Software, LLC https://www.graphpad.com/scientific- software/prism/ FlowJo v10.8 BD https://www.flowjo.com/solutions/flowjo Imaris 9.7.2 Bitplane https://imaris.oxinst.com/ Other Ficoll-Paque PLUS, density gradient media GE Healthcare Cat#17144003 CD8+ T cell isolation kit Miltenyi Biotech Cat#130-045-201 EGM-2 medium Promocell Cat#C-22011 EGM-2 supplement kit Promocell Cat#C-39216 Leukosep tubes, Negative Control:Article Title: The endothelial diapedesis synapse regulates transcellular migration of human T lymphocytes in a CX3CL1- and SNAP23-dependent manner. Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Swine Anti-Rabbit Immunoglobulins/HRP (affinity isolated), WB Dako Cat#P0399 Biological samples Fresh blood, in heparin tubes, from voluntary healthy donors Sanquin Blood Supply, Amsterdam, NL N/A Buffy coats, from the transfusion service Sanquin Blood Supply, Amsterdam, NL N/A Chemicals, peptides, and recombinant proteins Brefeldin A, BioXtra, for molecular biology Sigma Aldrich Cat#B6542 Recombinant human tumor necrosis factor a, TNF-a Peprotech Cat#300-01A; Accession Number: P01375 Pertussis toxin from Bordetella pertussis Merck, formerly from Sigma Aldrich Cat#P7208 Phalloidin Alexa Fluor 488 Thermo Fisher Scientific Cat# A12379 VybrantTM DiD Cell-Labeling Solution Thermo Fisher Scientific Cat#V22887 HOECHST 33342 Thermo Fisher Scientific Cat#H1399 Critical commercial assays Proteome profiler human chemokine kit Biotechne, RnD Systems Cat#ARY017 Experimental models: Cell lines HUVEC, human umbilical vein endothelial cells, pooled Lonza, Bornem, Beligum Cat#C2519A Oligonucleotides Human SNAP23, (NM_003825, NM_ 130798), siRNA1 Qiagen GeneGlobeID: SI00056448 Human SNAP23, (NM_003825, NM_ 130798), siRNA2 Qiagen GeneGlobeID: SI00056455 Negative Control siRNA, human Qiagen GeneGlobeID: 1022076 Recombinant DNA pmEGFP-N1-Lifeact Jaap van Buul, Sanquin, Amsterdam, NL N/A pLV-mScarlet_CAAX Jaap van Buul, Sanquin, Amsterdam, NL N/A pLV_ICAM-1_GFP Bram van Steen, Sanquin, Amsterdam, NL N/A Software and algorithms Fiji/ImageJ, ImageJ v1.53 (Schindelin et al., 2012) https://doi.org/10.1038/nmeth.2019 Graphpad Prism 9.1.0 (221) Graphpad Software, LLC https://www.graphpad.com/scientific- software/prism/ FlowJo v10.8 BD https://www.flowjo.com/solutions/flowjo Imaris 9.7.2 Bitplane https://imaris.oxinst.com/ Other Ficoll-Paque PLUS, density gradient media GE Healthcare Cat#17144003 CD8+ T cell isolation kit Miltenyi Biotech Cat#130-045-201 EGM-2 medium Promocell Cat#C-22011 EGM-2 supplement kit Promocell Cat#C-39216 Leukosep tubes, Software:Article Title: The endothelial diapedesis synapse regulates transcellular migration of human T lymphocytes in a CX3CL1- and SNAP23-dependent manner. Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Swine Anti-Rabbit Immunoglobulins/HRP (affinity isolated), WB Dako Cat#P0399 Biological samples Fresh blood, in heparin tubes, from voluntary healthy donors Sanquin Blood Supply, Amsterdam, NL N/A Buffy coats, from the transfusion service Sanquin Blood Supply, Amsterdam, NL N/A Chemicals, peptides, and recombinant proteins Brefeldin A, BioXtra, for molecular biology Sigma Aldrich Cat#B6542 Recombinant human tumor necrosis factor a, TNF-a Peprotech Cat#300-01A; Accession Number: P01375 Pertussis toxin from Bordetella pertussis Merck, formerly from Sigma Aldrich Cat#P7208 Phalloidin Alexa Fluor 488 Thermo Fisher Scientific Cat# A12379 VybrantTM DiD Cell-Labeling Solution Thermo Fisher Scientific Cat#V22887 HOECHST 33342 Thermo Fisher Scientific Cat#H1399 Critical commercial assays Proteome profiler human chemokine kit Biotechne, RnD Systems Cat#ARY017 Experimental models: Cell lines HUVEC, human umbilical vein endothelial cells, pooled Lonza, Bornem, Beligum Cat#C2519A Oligonucleotides Human SNAP23, (NM_003825, NM_ 130798), siRNA1 Qiagen GeneGlobeID: SI00056448 Human SNAP23, (NM_003825, NM_ 130798), siRNA2 Qiagen GeneGlobeID: SI00056455 Negative Control siRNA, human Qiagen GeneGlobeID: 1022076 Recombinant DNA pmEGFP-N1-Lifeact Jaap van Buul, Sanquin, Amsterdam, NL N/A pLV-mScarlet_CAAX Jaap van Buul, Sanquin, Amsterdam, NL N/A pLV_ICAM-1_GFP Bram van Steen, Sanquin, Amsterdam, NL N/A Software and algorithms Fiji/ImageJ, ImageJ v1.53 (Schindelin et al., 2012) https://doi.org/10.1038/nmeth.2019 Graphpad Prism 9.1.0 (221) Graphpad Software, LLC https://www.graphpad.com/scientific- software/prism/ FlowJo v10.8 BD https://www.flowjo.com/solutions/flowjo Imaris 9.7.2 Bitplane https://imaris.oxinst.com/ Other Ficoll-Paque PLUS, density gradient media GE Healthcare Cat#17144003 CD8+ T cell isolation kit Miltenyi Biotech Cat#130-045-201 EGM-2 medium Promocell Cat#C-22011 EGM-2 supplement kit Promocell Cat#C-39216 Leukosep tubes, Cell Isolation:Article Title: The endothelial diapedesis synapse regulates transcellular migration of human T lymphocytes in a CX3CL1- and SNAP23-dependent manner. Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Swine Anti-Rabbit Immunoglobulins/HRP (affinity isolated), WB Dako Cat#P0399 Biological samples Fresh blood, in heparin tubes, from voluntary healthy donors Sanquin Blood Supply, Amsterdam, NL N/A Buffy coats, from the transfusion service Sanquin Blood Supply, Amsterdam, NL N/A Chemicals, peptides, and recombinant proteins Brefeldin A, BioXtra, for molecular biology Sigma Aldrich Cat#B6542 Recombinant human tumor necrosis factor a, TNF-a Peprotech Cat#300-01A; Accession Number: P01375 Pertussis toxin from Bordetella pertussis Merck, formerly from Sigma Aldrich Cat#P7208 Phalloidin Alexa Fluor 488 Thermo Fisher Scientific Cat# A12379 VybrantTM DiD Cell-Labeling Solution Thermo Fisher Scientific Cat#V22887 HOECHST 33342 Thermo Fisher Scientific Cat#H1399 Critical commercial assays Proteome profiler human chemokine kit Biotechne, RnD Systems Cat#ARY017 Experimental models: Cell lines HUVEC, human umbilical vein endothelial cells, pooled Lonza, Bornem, Beligum Cat#C2519A Oligonucleotides Human SNAP23, (NM_003825, NM_ 130798), siRNA1 Qiagen GeneGlobeID: SI00056448 Human SNAP23, (NM_003825, NM_ 130798), siRNA2 Qiagen GeneGlobeID: SI00056455 Negative Control siRNA, human Qiagen GeneGlobeID: 1022076 Recombinant DNA pmEGFP-N1-Lifeact Jaap van Buul, Sanquin, Amsterdam, NL N/A pLV-mScarlet_CAAX Jaap van Buul, Sanquin, Amsterdam, NL N/A pLV_ICAM-1_GFP Bram van Steen, Sanquin, Amsterdam, NL N/A Software and algorithms Fiji/ImageJ, ImageJ v1.53 (Schindelin et al., 2012) https://doi.org/10.1038/nmeth.2019 Graphpad Prism 9.1.0 (221) Graphpad Software, LLC https://www.graphpad.com/scientific- software/prism/ FlowJo v10.8 BD https://www.flowjo.com/solutions/flowjo Imaris 9.7.2 Bitplane https://imaris.oxinst.com/ Other Ficoll-Paque PLUS, density gradient media GE Healthcare Cat#17144003 CD8+ T cell isolation kit Miltenyi Biotech Cat#130-045-201 EGM-2 medium Promocell Cat#C-22011 EGM-2 supplement kit Promocell Cat#C-39216 Leukosep tubes, FACS:Article Title: The endothelial diapedesis synapse regulates transcellular migration of human T lymphocytes in a CX3CL1- and SNAP23-dependent manner. Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Swine Anti-Rabbit Immunoglobulins/HRP (affinity isolated), WB Dako Cat#P0399 Biological samples Fresh blood, in heparin tubes, from voluntary healthy donors Sanquin Blood Supply, Amsterdam, NL N/A Buffy coats, from the transfusion service Sanquin Blood Supply, Amsterdam, NL N/A Chemicals, peptides, and recombinant proteins Brefeldin A, BioXtra, for molecular biology Sigma Aldrich Cat#B6542 Recombinant human tumor necrosis factor a, TNF-a Peprotech Cat#300-01A; Accession Number: P01375 Pertussis toxin from Bordetella pertussis Merck, formerly from Sigma Aldrich Cat#P7208 Phalloidin Alexa Fluor 488 Thermo Fisher Scientific Cat# A12379 VybrantTM DiD Cell-Labeling Solution Thermo Fisher Scientific Cat#V22887 HOECHST 33342 Thermo Fisher Scientific Cat#H1399 Critical commercial assays Proteome profiler human chemokine kit Biotechne, RnD Systems Cat#ARY017 Experimental models: Cell lines HUVEC, human umbilical vein endothelial cells, pooled Lonza, Bornem, Beligum Cat#C2519A Oligonucleotides Human SNAP23, (NM_003825, NM_ 130798), siRNA1 Qiagen GeneGlobeID: SI00056448 Human SNAP23, (NM_003825, NM_ 130798), siRNA2 Qiagen GeneGlobeID: SI00056455 Negative Control siRNA, human Qiagen GeneGlobeID: 1022076 Recombinant DNA pmEGFP-N1-Lifeact Jaap van Buul, Sanquin, Amsterdam, NL N/A pLV-mScarlet_CAAX Jaap van Buul, Sanquin, Amsterdam, NL N/A pLV_ICAM-1_GFP Bram van Steen, Sanquin, Amsterdam, NL N/A Software and algorithms Fiji/ImageJ, ImageJ v1.53 (Schindelin et al., 2012) https://doi.org/10.1038/nmeth.2019 Graphpad Prism 9.1.0 (221) Graphpad Software, LLC https://www.graphpad.com/scientific- software/prism/ FlowJo v10.8 BD https://www.flowjo.com/solutions/flowjo Imaris 9.7.2 Bitplane https://imaris.oxinst.com/ Other Ficoll-Paque PLUS, density gradient media GE Healthcare Cat#17144003 CD8+ T cell isolation kit Miltenyi Biotech Cat#130-045-201 EGM-2 medium Promocell Cat#C-22011 EGM-2 supplement kit Promocell Cat#C-39216 Leukosep tubes, |